Cells, Free Full-Text

High-resolution 3D images of organelles are of paramount importance in cellular biology. Although light microscopy and transmission electron microscopy (TEM) have provided the standard for imaging cellular structures, they cannot provide 3D images. However, recent technological advances such as serial block-face scanning electron microscopy (SBF-SEM) and focused ion beam scanning electron microscopy (FIB-SEM) provide the tools to create 3D images for the ultrastructural analysis of organelles. Here, we describe a standardized protocol using the visualization software, Amira, to quantify organelle morphologies in 3D, thereby providing accurate and reproducible measurements of these cellular substructures. We demonstrate applications of SBF-SEM and Amira to quantify mitochondria and endoplasmic reticulum (ER) structures.

Cell-free Fetal DNA — A Trigger for Parturition

Rapid cell-free forward engineering of novel genetic ring oscillators

Sequencing of Circulating Cell-free DNA during Pregnancy

Antibodies, Free Full-Text

Cell-free synthetic biology: Engineering in an open world - ScienceDirect

MitoQuicLy: A high-throughput method for quantifying cell-free DNA from human plasma, serum, and saliva - ScienceDirect

Cells, Free Full-Text

Serial Number Alcohol 120 1.9 6 - Colaboratory

Cell-free expression and synthesis of viruses and bacteriophages: applications to medicine and nanotechnology - ScienceDirect

Cells, Free Full-Text

Lactobacillus delbrueckii ssp. bulgaricus B-30892 can inhibit cytotoxic effects and adhesion of pathogenic Clostridium difficile to Caco-2 cells, Gut Pathogens