cKBET: assessing goodness of batch effect correction for single-cell RNA-seq

lt;p>Single-cell RNA sequencing reveals the gene structure and gene expression status of a single cell, which can reflect the heterogeneity between cells. However, batch effects caused by non-biological factors may hinder data integration and downstream analysis. Although the batch effect can be evaluated by visualizing the data, which actually is subjective and inaccurate. In this work, we propose a quantitative method cKBET, which considers the batch and cell type information simultaneously. The cKBET method accesses batch effects by comparing the global and local fraction of cells of different batches in different cell types. We verify the performance of our cKBET method on simulated and real biological data sets. The experimental results show that our cKBET method is superior to existing methods in most cases. In general, our cKBET method can detect batch effect with either balanced or unbalanced cell types, and thus evaluate batch correction methods.</p>

A benchmark of batch-effect correction methods for single-cell RNA sequencing data

A benchmark of batch-effect correction methods for single-cell RNA sequencing data, Genome Biology

Massively parallel digital transcriptional profiling of single cells

CellMixS: quantifying and visualizing batch effects in single-cell RNA-seq data

CellMixS: quantifying and visualizing batch effects in single-cell RNA-seq data

PDF] Projected t-SNE for batch correction

PDF) Batch effects and the effective design of single-cell gene expression studies

cKBET: assessing goodness of batch effect correction for single-cell RNA-seq

A benchmark of batch-effect correction methods for single-cell RNA sequencing data, Genome Biology